In Tim Burton's 1989 blockbuster Batman, David Baxt portrayed Thomas Wayne in his lone scene. The Waynes are stalked by Jack Napier, the future Joker and his partner. They are then murdered by Napier, rather than by Joe Chill. Napier then points the gun at Bruce and asks "Tell me kid, you ever dance with the devil by the pale moonlight?" Napier quickly abandons the crime scene when his partner calls for Jack. Martha's pearls falling into the gutter is prominently featured in slow motion. The last words Napier says to young Bruce are darkly ironic: "See you around kid." as Batman will later do battle with Napier and learns the real killer's identity years later when the Joker says to Bruce Wayne "Tell me my friend, you ever dance with the devil by the pale moonlight?" when he is about to shoot him in Vicki Vale's apartment.

Rather than use footage from the first movie, the third film in the series features a new flashback to the murder of the Waynes, with Ramsey Ellis as the young Wayne, Michael Scranton as Thomas Wayne, Eileen Seeley as Martha Wayne, and David U. Hodges as Jack Napier.